Sexually Transmitted Infections (STI) & HIV Testing

Drop-in clinics operate on a first come first served basis. Public Health reserves the right to limit the number of clients during these clinics. You can also receive confidential STI testing at your family doctor's office.

You can access our services through the drop-in clinic, booked ahead or same day appointments. We start taking calls at 8:30 am for same day appointments. Please call us at 519-883-2267!

Testing is the only way to know for certain if you have a sexually transmitted infection (STI). Some STIs do not cause symptoms and you could pass them on if you don't know you are infected.

Our clinics provide testing, treatment, education and referrals for sexually transmitted infection, including HIV testing. All services are free and confidential. No health card is required. You can make an appointment by calling 519-883-2267 or go to a drop-in clinic. The hours are shown below.

We also offer:

  • Access to birth control methods and pap tests (for 24-year olds and under)
  • Follow-up on post-therapeutic abortion
  • Treatment for genital warts (HPV)

Region of Waterloo Public Health clinics are LGBTQ (Lesbian, Gay, Bisexual, Transgender and Queer/Questioning) positive; sex trade workers are welcome.

Note: Hepatitis B vaccine is available free to individuals with sexual and/or needle-sharing risk factors. Free condoms are also available. 

If you've experienced a sexual assault, you have the following options:

  • Call the Sexual Assault Support Centre of Waterloo Region at 519-741-8633
  • Go to the emergency department of either St. Mary's Hospital in Kitchener, or Cambridge Memorial Hospital in Cambridge
  • Call the Sexual Assault Domestic Violence Treatment Centre (located at both St. Mary's and Cambridge Memorial hospitals) at 519-749-6994
  • Call the Police at 911

Appointments and drop-in services



 Drop-in Hours





99 Regina Street South 2nd Floor


Tuesday & Thursday
2:30 - 6:30 pm

9 am - 4 pm

9 - 4 pm




150 Main Street
1st Floor (rear of building)


1:30 - 6 pm

10 am - 1 pm

12 - 4 pm 



Youth-only Clinic

Teen Drop-in Zone

258 Hespeler Road


3:30 - 6:30 pm






Youth-only Clinic

Downtown Community Centre
35-B Weber Street West 


 Wednesday 3:30 - 6:30 pm



ACCKWA - HIV Testing Only

#203 637-614 King Street West (corner of King Street West and Wellington)


4 - 7 pm


Harm Reduction and Needle Syringe Program

Needle syringe services are available at all public health locations.  For more information and for a map of these locations, please check our Harm Reduction and Needle Syringe Program page.

Sexual Health Ontario

A condom broke - what should you do? Is it a cold sore or something else? If you have questions, Ask an Expert at the Sexual Health Ontario website.