Sexually Transmitted Infections (STI) & HIV Testing

Dont like waiting weeks for an appointment at the Sexual Health Clinic and for sexual health counselling, or to see a nurse in a drop-in clinic?

Try booking a same day appointment - call 519-883-2267!

Testing is the only way to know for certain if you have a sexually transmitted infection (STI). Some STIs do not cause symptoms and you could pass them on if you don't know you are infected.

Our clinics provide testing, treatment, education and referrals for sexually transmitted infection, including HIV testing. All services are free and confidential. No health card is required. You can make an appointment by calling 519-883-2267 or go to a drop-in clinic. The hours are shown below.

We also offer:

  • Access to birth control methods and pap tests (for 24-year olds and under)
  • Follow-up on post-therapeutic abortion
  • Treatment for genital warts (HPV)

Region of Waterloo Public Health clinics are LGBTQ (Lesbian, Gay, Bisexual, Transgender and Queer/Questioning) positive; sex trade workers are welcome.

Note: Hepatitis B vaccine is available free to individuals with sexual and/or needle-sharing risk factors. Free condoms are also available.

If you've experienced a sexual assault, you have the following options:

  • Call the Sexual Assault Support Centre of Waterloo Region at 519-741-8633
  • Go to the emergency department of either St. Mary's Hospital in Kitchener, or Cambridge Memorial Hospital in Cambridge
  • Call the Sexual Assault Domestic Violence Treatment Centre (located at both St. Mary's and Cambridge Memorial hospitals) at 519-749-6994
  • Call the Police at 911

Appointments and drop-in services




 99 Regina Street South
2nd Floor

 150 Main Street
1st Floor (rear of building)

 ACCKWA - HIV Testing Only
#203 637-614 King St. W. (corner of King St W. and Wellington)




 Drop-in Hours:

Tuesday & Thursday
2:30 pm - 6:30 pm

 Drop-in Hours:


1:30 pm - 6:30 pm

Drop-in Hours:


4-7 pm  

By Appointment:

Tuesday & Wednesday

9 am - 5 pm


12 pm - 7 pm 

 By Appointment:


10 am - 1 pm


12 pm - 4 pm

 By Appointment:





Drop-in clinics operate on a first come first served basis. Public Health reserves the right to limit the number of clients during these clinics.

Needle exchange

Needle exchange services are available at all public health locations.  For more information and for a map of these locations, please check our Needle Exchange page.

Sexual Health Ontario

A condom broke what should you do? Is it a cold sore or something else? If you have questions, Ask an Expert at the Sexual Health Ontario website.


Region of Waterloo Public Health
General Inquiries
99 Regina Street South, Waterloo, Ontario N2J 4V3

Phone: 519-575-4400
TTY: 519-575-4608

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Link to Region of Waterloo Public Health Home Page